Fig. 4. CycA and CycB are misregulated in twin. Anterior is leftwards in all panels. The pair in each row was stained and photographed in parallel. (A-D) CycA, red; fusome (1B1), green. (E,F) CycB, red; fusome, green. (A) Wild-type (twin/+) chamber showing a cyst in region 1 expressing CycA. Some of the CycA co-localized with the fusome, which appears yellow. Posterior to this cyst are several highly branched fusomes. (B) twin^S1/Df germarium showing very strong CycA staining throughout. CycA colocalizes with some fusomes even towards the posterior of the germarium (yellow). Fusomes are present, and contain branches. (C) Wild-type (twin/+) germarium showing CycA in two cysts in region 1. One of these cysts colocalizes CycA to the fusome. (D) twin^ry3/Df germarium showing CycA staining throughout. The misexpression phenotype is not as prominent as in the strongest allele, S1. The fusomes are clearly visible and highly branched. (E) Wild-type (twin/+) germarium showing CycB in two cysts in region 1. Both of these cysts show CycB co-localizing with the fusome. (F) twin^S1/Df showing very strong CycB staining throughout germarium. The unstained regions are sites of dying cysts. CycB colocalizes with the fusome in all the cysts. Staining with anti-CycE antisera did not reveal a difference between twin/Df and twin/+ germaria (not shown).