Fig. 5. Movement tracks of exemplary cells within the shield region of
e-cadherin morphant embryos during early stages of gastrulation.
(A-C) Lateral (sagittal) views of the shield at 6 hpf±0 minutes (A),
+52.7 minutes (B) and +105.5 minutes (C). (D-F) Tracks of the three exemplary
cells marked yellow in A-C. (G-I) Lateral (sagittal) views of the shield at
7.5 hpf±0 minutes (G), +18.5 minutes (H) and +40 minutes (C). (J-L)
Tracks of the three exemplary cells marked yellow in G-I. The cells were
tracked in 2.6-minute time intervals for a total period of 105 (cells 1-3) and
63 minutes (cells 4-6). For each exemplary cell, at least 10 other cells at
similar positions within the germ ring from at least three independent movies
were tracked. The blue tracks in D-F and J-L delineate the movement within the
embryo, while the red tracks show the movement of the respective cell within
the germ ring (for details see Materials and methods and Fig. S1 in
supplementary material). The yellow dots in D-F,J-L indicate the starting
points of the cell tracks. Scale bar in A: 30 µm.