Fig. 7. Cellular morphology and position of anterior mesendodermal (prechordal plate) cells in wild-type, e-cadherin morphant and mosaic embryos at early and late stages of gastrulation. (A,B) Face- on view at the anterior edge of the prechordal plate of wild-type (A) and e-cadherin morphant (B) embryos at 6.5 hpf. (C,D) Three-dimensional reconstruction of a transplanted wild-type (C) and e-cadherin morphant (D) cell within the anterior prechordal plate of a wild-type embryo at 7 hpf. (E,F) Three-dimensional reconstruction of a transplanted e-cadherin morphant (E) and wild-type (F) cell within the prechordal plate of an e-cadherin morphant embryo at 7 hpf. (G,H) Position of transplanted wild-type (green) and e-cadherin morphant (red) cells within the prechordal plate of a wild-type embryo at 10 hpf; lateral view with anterior to the left (G), and animal view with anterior to the top (H). The inset in G shows the position of the transplanted cells (yellow) within the shield directly after the transplantation, with dorsal to the right. For all transplantation experiments (C-H), cells were transplanted at 6 hpf and the distribution (mixing) of the different cell types was monitored straight after the transplantation. MO, e-cadherin morphant cells; wt, wild-type cells. Scale bars: in A, 20 µm; in C, 10 µm; in G, 200 µm.