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Fig. 1. Transient expression of robo2 mRNA in the developing olfactory placode, shown by whole-mount in situ hybridization. (A-E) Dorsal views with anterior to the top; (F) ventral view with anterior to the top. As the olfactory placode moves from a dorsal to a more ventral location during development, it is more easily viewed from the ventral side at 48 hpf. Arrowheads (both open and closed) denote the position of the olfactory placode. robo2 mRNA was detected in the olfactory placode in embryos at stages between 20-36 hpf (closed arrowheads in B-E). Scale bar: 50 µm.