Fig. 6. Ubiquitous misexpression of Slit2 causes posterior pathfinding errors of OSN axons. (A) Schematic of the experimental procedure. (B) A heterozygous hsp:slit2-gfp transgenic embryo overexpressing a Slit2-GFP fusion protein (green) and wild-type siblings at 24 hpf, which had received heat-shock treatment at 19 hpf. (C-H) One wild-type and two hsp:slit2-gfp/+ examples are shown. In Slit2-overexpressing embryos, some axonal fibers are deviated from the normal pathway and extend posteriorly (arrowheads and arrows in E-H), occasionally crossing the midline (arrows in E,F). C,E,G, frontal views with dorsal to the top; D,F,H, dorsal views with anterior to the left. Scale bar: 100 µm.