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Fig. 6. Apoptosis is not enhanced by caNotch and Cre. We performed TUNEL assays for the E17.5 brain after double electroporation of pCAG-ExNotchZ at E13.5 and pXCANCre at E15.5. The areas of the double-transfected hemisphere (B,C) and the opposite non-transfected hemisphere (A) of an electroporated brain are shown. Green signals show EYFP fluorescence. All three panels show the same section; A and C are merged views. Similarly, no increase of apoptosis was observed at E16.5 (data not shown). Scale bar: 200 µm.