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Fig. 3. The expression of Mkp3 over time correlates with differential levels of phosphorylated ERK in response to FGF bead implants. (A-C) Whole-mount in situ hybridisation of Mkp3 in chick embryos with FGF bead grafts, harvested after a 1-hour (A), 5-hour (B) or 24-hour exposure (C). (D) Western blot of untreated somites (-) or somites exposed to an FGF4 bead (+), harvested at the times indicated and probed with anti-phospho-ERK and {alpha}-tubulin antibodies. (E) scleraxis was induced by an FGF4 bead after 5 hours. (F,G) Sections demonstrating the distribution of ectopic transcripts after bead grafting, (F) Mkp3 and (G) scleraxis. Red asterisks in C,E indicate the bead.