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Fig. 5. Functional analysis of several genes in group 4. (A) Eight-cell stage
embryos were treated with or without SB203580 or SP600125 for 1 hour, and
stained with anti-CDX1 antibody or anti-Dkk1 antibody. Fluorescence was viewed
with a confocal microscope. (B) Effect of siRNA-based inhibition of genes in
group 4. One-cell stage embryos were injected with dsRNA oligonucleotides as
indicated, and cultured for 3 days. The numbers of morphologically normal or
abnormal embryos were counted, and embryos were stained with anti-Dkk1
antibody. Bright-field (upper) and fluorescence (lower) micrographs of
DDW-injected (left) and siCdx1/siDkk1/siFoxq1/siSox7-injected (middle and
right) embryos are shown.
