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Fig. 4. Avascular areas of the retina are hypoxic. EF5 staining (red) visualizes hypoxia in retinal wholemounts from P6 mice raised in normal atmosphere (A,B) and 80% oxygen (C,D). Blood vessels were stained with anti-collagen type IV antibody (green staining in A,C). Panels B and D are identical to A and C but with the green channel omitted. The arrow in D indicates a small, circular normoxic area in the centre of the retina.