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Fig. 3. Effect of DjPum RNAi on regeneration in D. japonica. Brightfield images of the injected organisms. Dorsal view, anterior is towards the top. Broken yellow lines indicate the blastema region. (A) A head fragment injected with DjPum dsRNA. (B) A water-injected head fragment, 4 days after the second transection. (C) A tail fragment injected with DjPum dsRNA, 4 days after the second transection. (D) A water-injected tail fragment, 4 days after the second transection. (E) A head fragment injected with DjPum dsRNA, 14 days after the second transection. (F) A tail fragment injected with DjPum dsRNA, 14 days after the second transection. (G) Visualization of a comparative RT-PCR experiment in planarians injected with DjPum dsRNA, water and Djeya dsRNA. DjEF2 is used as an internal amplification control. Scale bars: 250 µm.