Fig. 6. Distribution of mitoses in D. japonica, as detected by anti-phospho-histone H3. Confocal projections of 40 sections taken at 0.9 µm intervals through 36 µm were obtained from whole-mount preparations. The H3P signal is visualized in red. (A) Schematic drawing of a planarian. Red box indicates the body region corresponding to the confocal images depicted in B-D. (B) H3P immunostaining in a water-injected control, 15 days after the first transection. (C) H3P staining in a DjPum dsRNA-injected planarian 15 days after the first transection. (D) H3P staining of an intact planarian, 15 days after X-ray irradiation. Scale bars: 50 µm.