Fig. 5. The morphology of the microtubule network at cycle 9 interphase when nuclei move to the cortex (A-C, shown at higher magnification in A'-C'), and astral microtubule morphology at cycle 9 metaphase (A''-C''). (A-A'') two cycB embryo; (B-B'') six cycB embryo; (C-C'') sse^13m/six cycB embryo. These embryos were stained with an antibody against tubulin to label microtubules and with rhodamine-conjugated anti-histone H1 antibody to label nuclei for precise staging (not shown). Images in A-C and A'-C' are projections of 11 optical sections with a 1.5-µm interval. Images in A''-C'' are projections of six sections with 1-µm intervals. Note that interphase microtubules in the sse^13m/six cycB embryo (C,C') are stronger than those in two cycB embryos (A,A'), but the astral microtubules in metaphase sse^13m/six cycB embryo are not different from six cycB embryos (B'',C''). Scale bars: in C, 40 µm for A-C; in C', 20 µm for A'-C'; in C'', 10 µm for A''-C''.