Fig. 4. Molecular cloning of let-19 and dpy-22. Genetic maps of the let-19 (A) and dpy-22 (B) loci with rescuing cosmids. Structures of the genes and rescuing constructs are shown with the coding regions in gray and the Q-rich domain in dpy-22 hatched. The molecular lesions of the mutations are indicated. The sop-1-class mutations of dpy-22 are from Zhang and Emmons (Zhang and Emmons, 2000). The sy622 and sy655 mutations are from Moghal and Sternberg (Moghal and Sternberg, 2003). The total lengths of the protein products are indicated on the left. (C) Protein sequence comparisons of the C-terminal regions of MED13 homologs from C. elegans (Ce), human (Hs), mouse (Mm), rat (Rn), D. melanogaster (Dm), D. discoideum (Dd), S. pombe (Sp) and S. cerevisiae (Sc). The consensus sequence (Cons) is indicated in the top row. The numbers indicate positions in the complete peptide sequences. Black and gray backgrounds indicate identical or similar amino acids, respectively, in at least four aligned sequences. Amino acids considered similar are R/K/H, S/T, I/L/V/M, E/D, Q/N and F/Y/W. Stop signals are indicated by asterisks. The mutation site (R2834stop) of let-19(os36) is indicated in italics.