Fig. 8. Grafts of dorsal diencephalic tissue inhibit ZLI propagation. (A,B) Stage 13-14 dorsal diencephalic grafts were excised from a region containing Wnt3a-expressing cells (A), and intermediate telencephalic grafts from a region containing Foxg1-expressing cells (B). (C-F) Expression of Wnt3a (red, C) and Foxg1 (red, D) in relation to Shh (blue, D) after 2 days in culture. The propagation of Shh expression in the ZLI was inhibited adjacent to dorsal diencephalic grafts (E), but not in telencephalic controls (F). Dashed circles indicate grafted tissue.