Fig. 7. TAGteam sites in the zen ventral repression element (VRE) help activate transcription. (A) Schematic of transgenes showing the segments of the VRE that were used to drive an eve-lacZ reporter. (B-G) lacZ RNA in situ hybridization to cycle 14 embryos carrying the 600VRE (B), mut600VRE (C), 250VRE (D), 111VRE (E), mut111VRE (F), or (2X)111VRE (G) transgene. (H) Nuclear RNA hybridization dots in a cycle 7 embryo carrying the (2X)111VRE transgene, co-stained with DAPI for DNA to reveal nuclei. The arrow indicates a dot-containing nucleus, which is magnified in the inset. (I) Expression measured as the average percentage of dot-containing nuclei per embryo. Transcription onset is defined as the first cycle 5%. Six lines were examined for the mut111VRE construct, three for all others. For each nuclear cycle, 18 embryos were assayed for the 250VRE construct, and 37 for all others. Scale bars: B-G, 50 µm; H-J, 20 µm.