Fig. 2. Ventral anterior follicle cells adopt an appendage-producing fate in cic mutant egg chambers. BR-C expression was visualized in wild-type (A,B) and cic mutant (C-F) egg chambers using an antibody recognizing all BR-C isoforms (Emery et al., 1994). (A) Wild-type stage 10 egg chamber (dorsal view). (B) Wild type, stage 12, dorsal (top) and ventral (bottom) views. (C) Stage 12 cic^fetU6/cic^fetT6 egg chambers, dorsolateral (top) and ventrolateral (bottom) views. The high BR-C nuclei are more densely organized than those with low BR-C. (D) Stage 10B egg chamber (ventral view) with homozygous cic^fetU6 follicle cell clones marked by the absence of N-Myc (green). (E,E') Close-up of box I in D; clone boundary outlined in gray. In the vast majority of clones, ectopic elevated BR-C is cell autonomous. (F,F') Close-up of box II in D. Rare cases of nonautonomy (arrow, arrowhead) were observed near the endogenous appendage primordia. (G) Wild type, stage 12 (dorsal view), stained with rhodamine-labeled phalloidin. The cells of the appendage primordia are constricted apically. (H) Stage 12 ventral anterior cic^fetU6 follicle cell clones marked by the absence of N-Myc. (H') Rhodamine phalloidin staining of the clones in H reveals similar cell shape changes in cic mutant cells.