Fig. 3. cic mutant follicle cells exhibit ectopic mirr expression. (A-A'') Wild-type stage 10B egg chamber (dorsolateral view). (A) mirr-lacZ is expressed in dorsal anterior follicle cells. (A') At this stage, the two dorsal anterior high BR-C domains (outlined) have been established. (A'') Overlay illustrates that the mirr-lacZ domain aligns with the high BR-C domains. (B) Stage 10B cic mutant egg chamber, dorsal (top) and ventral (bottom) views. (C) Stage 10B (ventrolateral view). Homozygous cic^fetU6 follicle cell clones are marked by the absence of nuclear GFP. (C') Endogenous dorsal anterior (top arrow) and ectopic anterior mirr-lacZ expression in the egg chamber in C. Ectopic mirr-lacZ is observed in cells in the anterior half (arrowheads), but not in the posterior half (arrows), of the columnar epithelium. (D) Ventral cic^fetU6 mutant follicle cell clone (stage 10B) marked by the absence of N-Myc. (D') Ectopic mirr-lacZ expression is cell autonomous. (E-E'') Stage 14 egg chamber with a ventral cic^fetU6 mutant clone (E, arrow) exhibiting ectopic mirr-lacZ expression (E', arrow) and ectopic appendage material (E", arrow).