Fig. 4. Ectopic mirr expression is necessary and sufficient to induce appendage-producing fate. (A,A') Ventral anterior region of stage 11 cic^fetT6/cic^fetU6 egg chamber bearing follicle cell clones homozygous for mirr^e48 (A, absence of green). Ectopic high BR-C expression (A', red) is abolished in the mirr mutant cells. (B,B') Stage 14 egg chamber (lateral view) with follicle cell clones expressing UAS-mirr positively marked with GFP (green). BR-C expression (red) is also shown. (C,C') Stage 12 egg chamber (dorsolateral view) with positively-marked follicle cell clones expressing UAS-mirr (green). DE-Cadherin expression (red) was visualized with an anti-DE-cadherin antibody. (D,D') Stage 14 egg chamber (ventrolateral view) with homozygous fng¹³ follicle cell clones (D, absence of green). BR-C expression is shown in red.