Fig. 5. Ectopic mirr expression in cic mutant egg chambers is grk independent. Anterior is to the upper left in all panels. (A) cic mutant egg chamber heterozygous for grk (lateral view, dorsal to the upper right) exhibiting ectopic mirr-lacZ expression. (B) grk^2B6/grk^HK egg chamber heterozygous for cic. The exposure time has been increased to emphasize the lack of mirr-lacZ expression. (C) Stage 14 grk^2B6/grk^HK egg chamber exhibiting a typical lack of dorsal appendages. (D,D') Top and bottom focal planes of a grk^2B6/grk^HK; cic^fetU6/cic^fetT6 egg chamber. Uniform mirr-lacZ expression is observed around the anterior circumference. (E) Stage 14 grk^2B6/grk^HK; cic^fetU6/cic^fetT6 egg chamber. The eggshell lacks dorsal appendages but exhibits a pronounced collar of appendage material. (F-F'') cic^fetU6/cic^fetT6 egg chamber with homozygous rhomboid⁶ clones (ventral view). (F) rhomboid⁶ clones (lack of green) including most of the follicular epithelium. (F') The ectopic expression of high BR-C levels is unaffected. (F'') Merged image. Identical results were obtained with two different strong rhomboid alleles.