Fig. 7. GATA2 inhibits the proliferation of mouse embryonic neuroepithelial cells in culture. (A-F) Wild-type (A,B) and Gata2^–/– (C-F) embryonic mouse neuroepithelial cells (ENC); immunostaining with anti-GATA2 (A), anti-nestin (B,C, green), anti Sox2 (D, green) and anti-Tuj1/ßIII-tubulin (F, red) antibodies. Nuclei were stained with DAPI (blue) in B,C,E,F. Wild-type (B) or Gata2^–/– (C) ENCs express Nestin. Sox2 (D), but no Tuj1/ßIII-Tubulin (F), can be detected in Gata2^–/– cells, as in wild-type cells (not shown); D-F correspond to the same field. (G) Comparison of the percentage of serum-starved cells reaching the S phase at different time intervals after cell cycle release in the presence of serum and BrdU. (H) Comparison of the transcription level of cyclin D1, cyclin D2, cyclin D3 and cyclin E1, p27 and Notch1 genes by semi-quantitative RT-PCR. PCR products were separated on BEt agarose gels, then analysed and quantified with GelDoc camera and software (BioRad). Scale bar: 40 µm in A-C; 20 µm in D-F.