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Figure 4


Fig. 4. Expression of a dominant-negative BMP receptor in lateral mesoderm results in enhanced hematopoiesis. Two representative embryos are shown in A and B; views are lateral, anterior towards the top, with embryos still in their chorions. (A) Control embryos from the gata1:gfp transgenic line demonstrate the normal expression pattern of GFP in the ICM. (B) Embryos from the gata1:gfp transgenic line that had been injected at the one-cell stage with the I(lmo2{Delta}BR)I transgene and meganuclease. GFP expression is substantially increased in the ICM, compared with control embryos (arrowheads). (C) Dissociated cells were collected at the 16- to 17-somite stage from batches of control and transient transgenic embryos and analyzed by FACS to score quantitatively the numbers of GFP+ cells. Shown are results from one representative experiment, although the data were comparable in three independent experiments. Compared with control embryos (blue) the I(lmo2{Delta}BR)I transgenic embryos (red) show more than double the normal amount of GFP+ hematopoietic cells.