Fig. 1. Crz expression in the CNS during metamorphosis. (A,B) In situ hybridization of Crz mRNA. (A) Third-instar larval CNS, showing three pairs of DL neuron in the brain (Br) and eight pairs of vCrz neurons (brackets). (B) Pupal CNS at 36 hours APF. The number of neurons in the brain (arrowhead) is found to be six to eight per lobe, whereas none of vCrz neurons are detectable. (C,D) Crz-IR neurons in third-instar larval CNS (C) and pupal CNS at 12-24 hours APF (D). Consistent with the in situ hybridization results, no Crz-IR neurons are detectable in the VNC of the pupal CNS (arrow). At least five specimens were processed for each panel. (E) Colocalization (center) of Crz-gal4-induced GFP expression (left) in Crz-IR neurons (right). Scale bars: 100 µm.