Fig. 4. Pax7 is upregulated in regenerating tadpole tails. (A) RT-PCR shows that pax7 messenger RNA is more abundant in regenerating tails at 3 dpa, compared with the tails without amputation. (B) PCNA antibody staining (red) on parasagittal section of regenerating tail. DAPI (blue) shows the nuclei. The dense region in the centre is the notochord tip, with the blastema above and below. (C) Pax7 antibody-labelled cells (brown) on parasagittal sections of regenerating tail. Dorsal side upwards and anterior leftwards. Scale bars: 100 µm.