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Figure 3


Fig. 3. Aggregation of S2 cells induced by transfection with full-length and deleted ft and ds constructs. Expression in cells was induced by co-transfection with act-gal4. Cells in A-E were counterstained with rhodamine-labeled phalloidin (purple). (A) Control cells transfected with UAS-GFP (green) did not aggregate. (B,C) Cells co-transfected with UAS-ft, UAS-GFP and either UAS-ds (B) or UAS-ds{Delta}ICD (C) aggregated. (D,E) Cells co-transfected with UAS-GFP and either UAS-ft{Delta}ECD and UAS-ds (D) or UAS-ds{Delta}ECD and UAS-ft (E) did not aggregate. (F,G) Mixture of cells transfected with UAS-ds and UAS-GFP (green), and cells transfected with either UAS-ft (F, red) or UAS-ft {Delta}ICD (G, red) aggregated. Ft (anti-Ft, red and center panel) or Ft{Delta}ICD (anti-HA, red and center panel) concentrated with Ds (blue and right panels) at the sites of cell contact.