Fig. 5. The germ bands are dorsalized in sog RNAi embryos. (A) An At-sog RNAi embryo at stage 8 stained for DNA and At-otd transcripts. (B,C) Ventral views of untreated (B) and At-sog RNAi-treated (C) stage 8 embryos stained for pMad. (D-F) Flat preparations of untreated (D) and At-sog RNAi (E,F) stage 9 embryos stained for At-sog (red) and At-sim (purple). The arrow in E indicates the L1 segment, in which a patch of At-sog and At-sim expression intervenes between separated limb buds. (G-I) Flat preparations of untreated (G) and At-sog RNAi (H,I) late stage 9 embryos stained for At-sog (red) and At-pros (purple). Arrows in G indicate dorsal-most neural cell clusters. (J-Q) Prosomal (J,L,N,P) and opisthosomal (K,M,O,Q) regions of untreated (J,K,N,O) and At-sog RNAi (L,M,P,Q) stage 9 embryos stained for At-omb (J-M) or At-twi (N-Q) transcripts. a, anterior; p, posterior; Ch, chelicerae; Pp, pedipalps; L1-L4, first to fourth walking legs; sog Ri, At-sog RNAi. If not specified, anterior is to the top. Scale bars: 100 µm.