Fig. 4. (A) Requirement of STAT3 for inhibition of neurogenesis in vivo. Vectors encoding GFP alone (Control) or Cre recombinase plus GFP (Cre) were injected into the lateral ventricle of STAT3^flox/flox mice at E14.5. After 2 days, the fate of the GFP-positive cells was examined by immunohistochemistry with anti-GFP, anti-ßIII-tubulin (TuJ1), anti-SOX2, anti-nestin, anti-Musashi and anti-MAP2, as indicated. The percentages of TuJ1-positive cells and SOX2-positive cells among total cells in the transfected VZ region (60 µm width from the ventricular surface) are shown. The boxed regions in A are shown enlarged in B. Arrows in B indicate TuJ1-positive cells and MAP2-positive cells co-expressed without GFP. Scale bars: 20 µm in A; 40 µm in B.