Fig. 3. Cell markers and proliferation of wild-type neural lineages in the larval central brain. Wild-type MARCM clones labelled with membrane-tethered CD8::GFP (A-I; white) and centrosomin-GFP for centrosomes (D,E; white dots). Neuroblasts are indicated by asterisks and closely associated GMCs by arrowheads. (A) GFP-labelling of an entire clone shows a single large neuroblast and its associated progeny of adult-specific neurons (ganglion cells; GCs) which send their neurite in a common bundle (cell body fibre tract; CBT). (B-I) Neuroblast and late born cells are shown, immunostained as indicated in each panel. Mitosis, detected by PH3 immunostaining, is always restricted to neuroblasts (B: metaphase; D: telophase) and GMCs (E: anaphase/telophase; F,G: prophase/metaphase). CycE is detectable in neuroblast from interphase (E) to telophase (D) and in terminally dividing GMCs at interphase (D), but not during terminal division (E, inset shows CycE only). Mira is polarised at the neuroblast cortex during mitosis forming a crescent at metaphase (B) that segregates in budding GMC at telophase (C). Uniform cortical Mira is detected in neuroblasts at interphase (F,I) or in GMCs during mitosis (F, inset shows Mira only). Both neuroblasts and GMCs show Grh in their nuclei (H), whereas Pros is detectable in all nuclei of GMCs and GCs but not in the nucleus of neuroblasts (G,H, inset shows Pros staining only). Elav is expressed exclusively in nuclei of GCs that do not express Mira (I). Genotypes: (A-C,F-G) hsFLP/+; FRT40A/FRT40A, tubP-GAL80^LL10; UAS-mCD8::GFP^LL6, UAS-nlslacZ^J312/tubP-GAL4^LL7. (D,E) hsFLP/UAS-cnn::GFP; FRT40A, UAS-mCD8::GFP^LL5/FRT40A, tubP-GAL80^LL10; tubP-GAL4^LL7/+.