Fig. 6. Ice is required for cell death induced by X-irradiation and inhibition of protein synthesis. (A-F) TUNEL-stained third instar wing discs of various genotypes. (A) Untreated wild type; (B) X-irradiated wild type; (C) untreated Ice¹; (D) X-irradiated Ice¹; (E) X-irradiated Ice^+2.4; Ice¹; (F) X-irradiated dcp-1^Prev1. (G) Wild-type hemocytes exposed to cyclohexamide died within 4 hours. Removal of zygotic Ice (Ice¹)prevented almost all this death, which was restored in the presence of a wild-type Ice rescue transgene (Ice¹,Ice^+2.4). Animals homozygous for Ice¹ lack zygotic but not maternal Ice. Animals homozygous for dcp-1^Prev1 lack both maternal and zygotic dcp-1.