Fig. 3. Zfp488 expression in oligodendrocytes of the postnatal CNS. In situ hybridization of transverse sections of postnatal cerebral cortex, cerebellum and longitudinal sections of optic nerves. (A) At the neonatal stage P7, Zfp488 expression (red arrows) is mainly confined to the spinal white matter. (B-D) Expression of Zfp488 (red arrows) is highly enriched in the white matter of the cerebral cortex (B), and the cerebellum (C) and the optic nerve (D) at P14. A small population of Zfp488-expressing cells in the gray matter of the spinal cord and the forebrain is also evident (A and B, black arrows). (E) Double in situ hybridization for Zfp488 (blue color) and Plp1 (brown color) shows that Zfp488-expressing cells (arrows) are co-labeled with Plp1 in the corpus callosum. Small arrowheads indicate a linear array of interfascicular oligodendrocytes expressing both Zfp488 and Plp1. (F) Double in situ hybridization for Zfp488 and Pdgfra in the P14 cerebral cortex shows that Zfp488-expressing cells (purple) are not co-labeled with Pdgfra (brown) as indicated by purple and brown arrows, respectively. SC, spinal cord; Fbn, forebrain; Cbm, cerebellum; OP, optic nerve; CC, corpus callosum; Ctx, cortex.