Fig. 8. Expression of Zfp488 is correlated with oligodendrocyte differentiation. (A) Expression of Zfp488 increased as oligodendroglial cells underwent differentiation. Primary rat OPCs were isolated from neonate rat at P2 and cultured in growth medium. Total RNA was harvested from cell lysates before and after switching to differentiation medium at the time indicated. Real-time RT-PCR was performed to determine the relative amounts of Zfp488, Cnp, Mbp and Nkx2.2 expression. (B) CG4 cells were analogously treated and analyzed by real-time RT-PCR as described above in A. (C) Cultured CG4 cells in growth medium were transfected with control GFP siRNA and Zfp488 siRNA (100 nM) for 48 hours. Total RNAs were isolated before and after siRNA transfection. Real-time PCR was performed to determine the expression level of Zfp488, Cnp, Mbp and Gapdh. All data were derived from three independent experiments and shown as mean±s.d. Gapdh gene expression was used as the internal control.