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Figure 2


Fig. 2. Cx43{alpha}1 modulation of neural crest cell motility on fibronectin. The speed and directionality of cell locomotion was quantitatively assessed using time lapse images of CNCs explants from Cx43{alpha}1KO and CMV43 embryos plated on different fibronectin coating densities. Images were captured every 10 minutes over a 20-hour interval. As fibronectin density increased, wild-type and nontransgenic control CNCs (clear bars) show increased directionality, but decreased speed of cell locomotion (A,B). However, Cx43{alpha}1KO cells failed to achieve high directionality, while their speed of cell locomotion decreased more rapidly (A). By contrast, CMV43 neural crest cells exhibited higher directionality and higher speed of cell locomotion at 1 and 15 µg/ml fibronectin, respectively (B). Numbers in bars indicate the total number of cells analyzed. Error bars indicate the s.e.m. *P<0.05; **P<0.01; ***P<0.001, compared with wild type. #P<0.05; ##P<0.01; ###P<0.001, compared with hemi- or heterozygotes.