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Figure 3


Fig. 3. Assays for fetal hematopoietic stem cell (HSC) development. (A) (Top panel) An E10.5-11 mouse embryo. HSCs can be reliably assayed only by transplanting them into myeloablated (e.g. irradiated) recipients (lower panel) and then documenting their ability to give rise to sustained (3-6 months) multilineage hematopoietic reconstitution. Functional adult-type HSCs that can achieve such reconstitution in this assay are found only in mouse embryos or extra-embryonic tissues after E10.5-11.5. (B) (Top panel) A mouse embryo at E9.0. HSC activity can be observed at a younger age, and higher hematopoietic chimerism from immature HSCs (pre-HSCs) can be obtained if more permissive recipients (e.g. newborn mice, Rag2-/- {gamma}c-/- mice, lower left) are used. Transplantation directly into the long bones of a recipient can also improve engraftment (lower right). Intermediate explant culture of pSP cells also allows detection of HSC activity at a younger age.