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Figure 4


Fig. 4. Depletion of CDC-37 affects the distribution of PAR-3 and PAR-6 differently. Each column shows multiple images from a wide-field microscopy time-lapse movie of a single embryo through the first cell cycle. Note that, in control embryos, cortical PAR-3::GFP (column 1) and GFP::PAR-6 (column 3) are co-localized and asymmetric, whereas the two proteins behave differently in cdc-37(RNAi) embryos (columns 2 and 4; see Results). The arrows point to the pericentriolar accumulation of GFP::PAR-6 in the cdc-37(RNAi) embryos. Scale bar: ~10 µM.