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Figure 5


Fig. 5. Distributions of PAR-6 and PKC-3 after single and double depletions of CDC-37 and anterior PAR proteins. All embryos in the immunofluorescence images are at late prophase or metaphase of the first cell cycle. (A-D) PAR-6 distribution (A,B) and PKC-3 distribution (C,D) in par-3(it71) and par-3(it71); cdc-37(RNAi) treated embryos. (E,F) PAR-6 localization in the pkc-3(RNAi) or pkc-3(RNAi); cdc-37(RNAi) background. (G,H) PKC-3 localization in par-6(zu222) or par-6(zu222); cdc-37(RNAi) treated embryos. The cytoplasmic particles in A, B and E are P granules that are detected with some preparations of affinitypurified anti-PAR-6 antibody.