Fig. 1. Perlecan is required for integrity of the follicle cell epithelium. (A) Schematic drawing of an ovariole and FCE. (B,B') Pcan accumulates in the BM after the FCE is formed and is expressed in the muscular sheath (asterisk). (C) Pcan stripes run perpendicular to the long axis of the egg chamber. Yellow lines indicate cell borders. The arrow indicates the orientation of the stripes. (D,D') In a trol clone (broken line) expression of Pcan is absent. Red, Pcan; green, GFP. (E) trol clones frequently loose their epithelial organization and become multilayered (arrows). Occasionally mutant cells can be found in aberrantly positioned between the germ cells (arrowhead). Green, GFP; red in E, DNA. Clones are marked by the absence of GFP.