Fig. 5. SoxE factors function redundantly at the neural plate border. (A) Sox8, Sox9 or Sox10 can equally rescue Slug expression levels at stage 14 and expand Sox10 expression domain in Sox8-depleted embryos. In these experiments, Sox10 expression was evaluated using a probe against Sox10 3' UTR. (B) Western blot analysis. Detection of SoxE-GR proteins in extracts from injected embryos collected at stage 17 after injection at the two-cell stage. The fusion proteins are expressed at similar levels, as revealed with -GR antibody. -, uninjected control embryo; -tubulin is presented as a loading control.