Fig. 7. Blocking ephrin A/EphA signalling in vitro led to some cC-VC axons turning caudally. cC-VC axons were anterogradely labelled on organotypically cultured stage 25-26 hindbrains. (A) Without PI-PLC, in 20/25 samples, all axons turned rostrally. (B) With PI-PLC, in 25/32 samples, some axons turned caudally (white arrow). (P<0.0001, Fisher's test). (C) With hFc, in 21/29 samples, all cC-VC axons turned rostrally. (D) With EphA3-Fc, in 17/27 samples, some cC-VC axons turned caudally (white arrow) (P<0.015, Fisher's test). Scale bar: 400 µm.