Fig. 7. Other GABAergic and non-GABAergic markers are present in the SC of Mgn^tZ/tZ embryos. In situ hybridization with Mgn, Pitx2 and Ebf2 probes in adjacent coronal (A-H) at E12.5, or with Gat1, Brn3a and Gad67 in sagittal (I-P) sections at E13.5 in wild-type and in Mgn^tZ/tZ embryos. (A,B,I,J) Bright-field images. No changes for Pitx2 (E,F) and Ebf2 (G,H) could be observed when comparing wild-type and mutant mice. Mgn was absent in the mutant (compare C with D). At E13.5, no changes in the expression of Gat1 (K,L) or Brn3a (M,N) were detectable in the SC of the mutant when compared with the wild type, whereas the expression of Gad67 is totally abolished in the SC of the homozygous mutant mice (P, compare with O; see arrows).