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Figure 3


Fig. 3. Sema3d knockdown inhibits cell cycle in the hindbrain. (A-D) Immunodetection in the hindbrain at 17 hpf of BrdU (A,B) and PH3 (C,D) in CONMO (A,C) and 3DMO (B,D) injected embryos. (E,F) Quantification of the number of BrdU (E) and PH3 (F) labeled nuclei in the neuroepithelium of rhombomeres 3-5. Numbers in parentheses indicate n. *P<10-4 compared with CONMO, t-test. (A-D) Dorsal views, anterior is leftwards. Scale bar: 40 µm for A-D.