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Figure 1


Fig. 1. Interaction between BicD and Polo in two-hybrid and in vivo. (A) Yeast two-hybrid interaction between Polo and the N-terminal part of BicD (amino acids 120-350). The whole Polo-Box domain [Polo-Boxes 1 and 2 (red) and a short helix represented in gray] but not the kinase domain (green) are required for the interaction. (B) Immunoprecipitation using anti-GFP antibodies (IP-GFP) from ovary extracts from wild-type (lane 1), GFP-Polo (lane 2) and Ubi-NlsGFP (lane 3) flies, showing BicD specifically coprecipitates with GFP-Polo (lane 2, IP-GFP).