Fig. 3. Polo localization is dependent on BicD, egl and the Dynein complex. (A) BicD^r5 germline clones marked by the absence of NlsGFP. (B) BicD^PA66 homozygous germarium. (C) egl^wu50/RC12 ovariole and (D) dmn^K16109 germline clones marked by the absence of GFP. In A to D, the first images show SCs (red, white in the second picture), GFP-Polo (green dots, white in the third picture) plus NlsGFP (green nuclei, white nuclei in the third picture) and Hts (blue) in A and D. Polo localization is not restricted in any of these different genotypes, and the meiosis is not maintained in any cell of the cyst (at least 50 mutant cysts were scored for each genotype). In BicD (A) and egl (C) null mutants, meiosis starts in all the cells in region 2a whereas in BicD^PA66 (B) and dmn (D) mutants normal initiation of meiosis in two to four cells is observed.