Fig. 5. Polo overexpression affects meiosis progression and oocyte differentiation. (A) UAS-Polo overexpression with a germline-specific driver leads to the formation of SCs (white) in more than four cells per cyst in region 2a, and to a delay in meiotic restriction in region 2b. (B) Overexpression of GFP-Polo. C(3)G (red, white in B'), BicD (blue, white in B''), and GFP-Polo (green, white in B'''). (B') Meiosis can initiate in more than four cells, and cysts in region 3 still contain four cells in meiosis, thus indicating a strong delay in meiosis restriction to one cell. (B'') Polo overexpression leads to a delay in BicD accumulation in the oocyte and localization to the posterior of the oocyte. (B''') Cysts from region 2a to region 3 contain an unusually high number of Polo dots that are not restricted to one cell. There is a correlation between cells containing the strongest Polo speckles and meiotic cells in the cyst in region 3.