Fig. 3. Design of tud transgenic constructs and their expression. (A) tud full-length and deletion constructs. Tud domains deleted in the constructs are shown as white squares. (B) Western blot detection of transgenic Tud proteins. Ovary extracts from the wild type, which expresses no transgenes (`Wild-type' and `-HA' lanes), and those from transgenic lines, each of which expresses one copy of the transgene in a tud¹/Df(tud) background, were used. Endogenous Tud protein and HA-Tud proteins were detected with TUD-A63 (Tud) and anti-HA (HA) antibodies, respectively. Dynein bands, detected with anti-Dynein antibody, served as loading controls. The full-length transgene is expressed at about 20% of the wild type level. mini-tud 2 and mini-tud 3 are expressed at similar levels to endogenous, wild-type Tud. Expression of the mini-tud 1 transgene was consistently weak (2-3% of the mini-tud 3 amount) in several transgenic lines (lines A-C), probably due to poor protein stability.