Fig. 3. Effects of overexpressing MIR167 genes. (A) Sequences of miR167 target sites on ARF6 and ARF8 mRNA, and the mutated target sites of mARF6 and mARF8. The Watson-Crick base pairings to the miR167 sequence are shown. G (kcal/mol) was calculated by Mfold (Zuker, 2003). Mutated nucleotides are in lower case. (B) Phenotypes of plants overexpressing four different MIR167 genes. From left to right: wild type (WT), arf6/+ arf8, arf6 arf8, P_35S::MIR167a (a), P_35S::MIR167b (b), P_35S::MIR167c (c) and P_35S::MIR167d (d). (C) Stage 13 flowers of P_35S::MIR167 plants. Genotypes are the same as in B. (D) Northern blot analyses of MIR167-overexpressing transgenic plant flowers. U6 snRNA and ß-tubulin are included as loading controls. Numbers beneath lanes indicate relative transcript levels normalized to loading controls.