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Figure 3


Fig. 3. Wnt stimulation induces a colocalization of Daam1 and Profilin1. (A) Epitope-tagged Daam1 (HA) or (B) endogenous Daam1 (red) colocalizes with GFP-Profilin1 to structures resembling actin stress fibers (arrow) in response to 3 hours Wnt3a but not control CM stimulation. Note in A and B, magnifications of the boxed areas (white) are shown below each respective panel. (C) Transfection of Profilin1 siRNA but not a control GFP siRNA reduces the level of endogenous Profilin1 in NIH3T3 cells. ß-catenin and Daam1 are used as loading controls. (D) Top-Flash assay reveals Wnt3a CM and Dishevelled but not control CM or {Delta}DIX-Dishevelled induces reporter activation in NIH3T3 cells. Transfection of control or Profilin1 siRNA does not interfere with reporter activation by either Wnt stimulation or Dishevelled expression.