Fig. 6. XProfilin1 is required for gastrulation. (A) Injection of Profilin1 RNA dorsally but not ventrally inhibits gastrulation with the resulting embryos having open neural folds and reduced anterior structures. (B) XProfilin1 Morpholino (MO) sequence and diagram of the Profilin1 rescue construct (N-Profilin1). (C) Injection of the XProfilin1 MO inhibits translation of Myc-tagged Profilin1 in a dose dependent way but does not affect the endogenous levels of Rho and Rac proteins. (D) Dorsal injection of XProfilin1 MO inhibits gastrulation and results in a similar gastrulation defect phenotype as overexpression of Profilin1 RNA (see A). This phenotype is reversed by injection of N-Profilin1 with the XProfilin1 MO. (E) Quantitation of the phenotypic results from overexpression or depletion of XProfilin1. Injections were performed into the dorsal or ventral marginal zone of the 4-cell embryo, and phenotypes were scored at the tadpole stage.