Fig. 5. Expression of cell-adhesion molecules in pseudoglandular structures of the 17.5-day knockout liver. Intrahepatic biliary epithelial cells (arrows) are negatively or weakly immunostained for 3 and ß4 integrin subunits in the wild-type liver (A,B) although extrahepatic bile duct cells are moderately immunostained for ß4 integrin subunits (B). E-cadherin expression is strong in biliary epithelial cells, but they have weak N-cadherin expression in the wild-type liver (arrows) (C,D). In the knockout liver, cells of periportal pseudoglandular structures moderately express both integrin subunits (arrowheads) (E,F). In addition, almost all cells of pseudoglandular structures strongly or moderately express E-cadherin and N-cadherin (G,H) but those around the portal vein are negative for N-cadherin (arrowhead, H). PV, portal vein. Scale bars: 50 µm.