(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. RhoGEF64C is a positive regulator of Rho1 and its mRNA and protein are apically localised. (A) In situ hybridisation for RhoGEF64C showing expression in the posterior spiracle primordium (black arrows) and hindgut (white arrowhead), during retraction of the germ band. (B) Staining for the apical RhoGEF64C (red) in GFP-Actin expressing spiracle cells (stage 15). (C,D) RhoGEF64C mRNA is apically localised, surrounding the lumen of the posterior spiracles (C, lateral view) and hindgut (D, dorsal view) (stage 15). (E) Deletion of the Dbl plus PH domain (GEF64C{Delta}Dbl) abrogates apical localisation of RhoGEF64C mRNA in the posterior spiracles, as opposed to truncations of the UTR regions (GEF64C FL ({Delta}5'UTR) and GEF64C {Delta}5'3'UTR); CDS - coding sequence. Dorsal views. (F) Expression of RhoGEF64C FL rescues the RhoN19-induced phenotype, as opposed to the truncated form RhoGEF64C{Delta}Dbl. (G) RhoGEF64C RNAi downregulates the expression of this gene, as assessed by in situ hybridisation. Notice the formation of irregular Filzkörpers with partially disrupted cortical Actin. Green, GFP-Actin. Scale bars: 50 µm in A; 10 µm in B-D,F,G; and 20 µm in E.