Fig. 2. Analysis of somatosensory TC projections in EphA4 mutants. (A-C) Analysis of somatosensory TC projections in mutants displaying abnormal EphA4 kinase activity. In EphA4^GFP/GFP (A), EphA4^EE/EE (B) and EphA4^KD/KD (C) mutants, similar arrays of ectopic cells can be found in the medial part of the VPM (arrows). (D,E) Analysis of somatosensory TC projections in mutants displaying EphA4 lacking non-catalytic functional domains. EphA4^PBM (D) and EphA4^SAM (E) mutants display a normal pattern of TC projections. Scale bar in A: 400 µm for A-E. In A,C,D,E, left panels show corresponding DiI injection sites in S1. (F) Summary of the penetrance of TC topographic defects found in the various EphA4 mutants analyzed. The first line shows the number of affected animals/number of tested animals. The second line shows the percentage of animals displaying defective TC mapping in the VB.