Fig. 3. Analysis of somatosensory TC projections in EphA4 heterozygote animals. (A-C) In some EphA4^WT/GFP (A), EphA4^WT/KD (B) and EphA4^WT/KO (C) mutants, arrays of ectopic cells can be found in the medial part of the VPM (arrows), as in corresponding homozygous mutants. Scale bar in C: 400 µm for A-C. (D) Expression of EphA4 in the thalamus of heterozygous EphA4 mutant mice. Protein (50 µg) from thalamic extracts of the indicated mutant mice was resolved by SDS-PAGE and analyzed by western blot using an anti-EphA4 antibody raised against the extracellular domain of the protein (upper panels); the blot was reprobed with an anti-tubulin antibody for loading control (lower panels). (E) Summary of the penetrance of TC topographic defects found in the various EphA4 heterozygote mutants analyzed. The first line shows the number of affected animals/number of tested animals. The second line shows the percentage of animals displaying defective TC mapping in the VB.